Bitter plant extract may suppress food intake

Melbourne: Scientists have identified a highly bitter plant extract that can suppress food intake by stimulating the secretion of gut peptide hormones involved in appetite regulation.

Gut chemosensory mechanisms, particularly those involved in detecting and relaying to the brain the chemical composition of food during digestion, play an important role in regulating appetite and food intake. The researchers including those from University of Auckland, New Zealand hypothesised that activation of specific bitter taste receptors which are expressed throughout the gastrointestinal tract by hormone secreting 'enteroendocrine' cells, could also regulate food intake by triggering the release of satiety or 'fullness' hormones, a mechanism termed as "bitter brake."

The team screened over 900 plant extracts for their ability to stimulate enteroendocrine "I cell" hormone release before identifying a highly bitter, non-nutritive plant derived ingredient they call "Amarasate extract" to take forward into clinical testing. Their aim was to establish the efficacy of the Amarasate extract to modify acute energy intake, subjective ratings of appetite and gut peptide hormone concentrations.

Twenty lean healthy male volunteers were recruited (mean body mass index 23.4 kilogramme per square metre with 19 completing all three treatments within the randomised, double-blind, placebo controlled cross-over study. On each of the three treatment days, overnight fasted participants were provided with a standardised megajoule (578 calorie) energy breakfast.

Treatments comprising 500 mg Amarasate extract or a placebo were administered in either gastric pH resistant or standard  hypromellose capsules for targeted intestinal (duodenal) or stomach (gastric) release, respectively. The site of action within the gut is a key part of the hypothesised "bitter brake" mechanism.

To maintain treatment blinding, placebo capsules were also administered as part of each treatment. Blood samples and subjective ratings of appetite were
taken throughout the day. This was followed by a minimum of one week rest ('washout') period between treatments. Researchers found that, compared with placebo, both gastric and duodenal delivery of the Amarasate extract stimulated significant increases in the gut peptide hormones CCK, GLP-1 and PYY while significantly reducing total (lunch plus snack) meal energy intake by 911 kJ (218 calories) and 944 kJ (226 calories), respectively.

However, no significant treatment effects were observed for any subjective ratings of appetite or nausea. "We have demonstrated that activation of the 'bitter
brake' mechanism by a bitter plant extract can stimulate the release of gut peptide hormones involved in appetite regulation and suppress subsequent feeding behaviour in healthy men," researchers said.